At present, our understanding of polarizing microscope is not very clear. Polarizing microscope is a kind of microscope to identify the optical properties of fine structures of substances. All birefringent substances can be distinguished clearly under the polarizing microscope. Of course, these substances can also be observed by staining, but some of them are impossible, so the polarizing microscope must be used.
The application of polarizing microscope can be said that it is widely used in mineral, chemical and other fields. In Botany, such as identification of fibers, chromosomes, spindles, starch grains, cell walls, and whether there are crystals in cytoplasm and tissues. It is a method to change the ordinary light into polarized light for microscopy, so as to identify whether a substance is monorefractive isotropy or birefringent anisotropy. In plant pathology, the invasion of pathogens often causes changes in the chemical properties of tissues, which can be differentiated by polarized light microscopy. In human and zoology, polarizing microscopy is often used to distinguish skeleton, tooth, cholesterol, nerve fiber, tumor cell, striated muscle and hair. Birefringence is the basic characteristic of crystal. Therefore, in biology, many structures also have birefringence, which needs to be distinguished by polarizing microscope.
I believe that through my introduction, I hope you can learn more about the characteristics and application of polarizing microscope.